The Edinburgh-based firm designs, manufactures and deploys rockets to clear the way for small satellite manufacturers looking to access Space.
It successfully conducted Scotland’s first commercial rocket launch by a private company two years ago.
Skyrora chief executive Volodymyr Levykin said the investment in Rosyth would bring 170 highly skilled jobs to the Kingdom over the next decade.
He said: “The opening of our engine test complex represents a giant leap forward for the UK’s ambitions as a space nation and Scotland’s status as a space hub.
“The location and additional jobs will benefit the UK space industry and help the overall economy grow.
“It will also allow Skyrora’s highly skilled workforce and a young generation of engineers and technicians to be a part of this space revolution.
“Skyrora has developed and come so far as a team and a company, and I am really proud to see how many milestones we have achieved in a short period of time.”